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Image Search Results
Journal: Nature biotechnology
Article Title: Spatially Informed Cell Type Deconvolution for Spatial Transcriptomics
doi: 10.1038/s41587-022-01273-7
Figure Lengend Snippet: (A) The UMI counts of Slide-seq V2 data (right panel) displays the structure and the shape of hippocampus tissue, highly consistent with the image from Allen Reference Atlas (left panel) (B) The dominant cell type on each location inferred from four different deconvolution methods. Compared deconvolution methods include MuSiC, RCTD, SPOTlight, spatialDWLS, stereoscope and CARD. (C) Top panels display on each spatial location the proportion of each of the cell types inferred by CARD. Bottom panels display the expression levels of corresponding cell type specific marker genes. The examined cell types are CA1 cells, CA3 cells and dentate cells. (D) Bar plots display the comparisons of the mean gene expression level of marker genes in the major regions inferred by different deconvolution methods; (E) CARD imputes gene expression for four marker genes on a fine grid set of spatial locations, resulting in a refined spatial map of gene expression. (F) The proportion of each of the cell types on each location inferred by CARD in the 10x Visium dataset. (G) The expression levels of corresponding cell type specific marker genes in the 10x Visium dataset.
Article Snippet: In addition, modeling spatial correlation allows CARD to impute cell type compositions as well as gene expression levels on new locations of the tissue, facilitating the construction of a refined spatial map with an arbitrarily high resolution for any spatial transcriptomics technologies -- both these features are in direct contrast to a recent method BayesSpace that can only enhance
Techniques: Expressing, Marker, Gene Expression